A humanized mouse identifies the bone marrow as a niche with low therapeutic IgG activity

Lux A, Seeling M, Baerenwaldt A, Lehmann B, Schwab I, Repp R, Meidenbauer N, Mackensen A, Hartmann A, Heidkamp GF, Dudziak D, Nimmerjahn F (2014)

Publication Type: Journal article

Publication year: 2014

Journal

Cell Reports Elsevier (Cell Press)

Book Volume: 7

Pages Range: 236-48

Journal Issue: 1

DOI: 10.1016/j.celrep.2014.02.041

Abstract

Genetic differences between humans and in vivo model systems, including mice and nonhuman primates, make it difficult to predict the efficacy of immunoglobulin G (IgG) activity in humans and understand the molecular and cellular mechanisms underlying that activity. To bridge this gap, we established a small-animal model system that allowed us to study human IgG effector functions in the context of an intact human immune system without the interference of murine Fc? receptors expressed on mouse innate immune effector cells in vivo. Using a model of B cell depletion with different human IgG variants that recognize CD20, we show that this humanized mouse model can provide unique insights into the mechanism of human IgG activity in vivo. Importantly, these studies identify the bone marrow as a niche with low therapeutic IgG activity.

Authors with CRIS profile

Anja Lux Lehrstuhl für Genetik Michaela Seeling Lehrstuhl für Genetik Birgit Lehmann Lehrstuhl für Genetik Andreas Mackensen Lehrstuhl für Innere Medizin V Arndt Hartmann Lehrstuhl für Allgemeine Pathologie und Pathologische Anatomie Gordon Frederik Heidkamp Department of Dermatology Diana Dudziak Professur für die Biologie Dendritischer Zellen Falk Nimmerjahn Lehrstuhl für Genetik

Involved external institutions

Klinikum am Bruderwald DE Germany (DE)

How to cite

APA:

Lux, A., Seeling, M., Baerenwaldt, A., Lehmann, B., Schwab, I., Repp, R.,... Nimmerjahn, F. (2014). A humanized mouse identifies the bone marrow as a niche with low therapeutic IgG activity. Cell Reports, 7(1), 236-48. https://doi.org/10.1016/j.celrep.2014.02.041

MLA:

Lux, Anja, et al. "A humanized mouse identifies the bone marrow as a niche with low therapeutic IgG activity." Cell Reports 7.1 (2014): 236-48.

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