Transduction of Herpesvirus saimiri-Transformed T Cells with Exogenous Genes of Interest

Martínez-Barricarte R, De Jong SJ, Markle J, De Paus R, Boisson-Dupuis S, Bustamante J, Van de Vosse E, Fleckenstein B, Casanova JL (2016)

Publication Type: Journal article

Publication year: 2016

Journal

Current Protocols in Immunology Wiley

Book Volume: 115

Pages Range: 7.21C.1-7.21C.12

DOI: 10.1002/cpim.15

Abstract

Human T cells can be transformed and expanded with herpesvirus saimiri (HVS). HVS-transformed T cells from patients have facilitated the study of a broad range of primary immunodeficiencies (PID) in which T-cell development or function is altered. However, the utility of HVS-transformed T cells for genetic studies has been limited by technical challenges in the expression of exogenous genes, including wild-type or mutant alleles. A novel, gamma retrovirus-based method for the simple and reliable transduction, purification, and study of HVS-transformed T cells is described. © 2016 by John Wiley & Sons, Inc.

Authors with CRIS profile

Bernhard Fleckenstein Medizinische Fakultät

Additional Organisation(s)

Institute of Clinical and Molecular Virology

Involved external institutions

Leiden University Medical Center NL Netherlands (NL) Hôpital Necker-Enfants malades FR France (FR)

How to cite

APA:

Martínez-Barricarte, R., De Jong, S.J., Markle, J., De Paus, R., Boisson-Dupuis, S., Bustamante, J.,... Casanova, J.L. (2016). Transduction of Herpesvirus saimiri-Transformed T Cells with Exogenous Genes of Interest. Current Protocols in Immunology, 115, 7.21C.1-7.21C.12. https://doi.org/10.1002/cpim.15

MLA:

Martínez-Barricarte, Rubén, et al. "Transduction of Herpesvirus saimiri-Transformed T Cells with Exogenous Genes of Interest." Current Protocols in Immunology 115 (2016): 7.21C.1-7.21C.12.

BibTeX: Download