Intracellular African swine fever virus DNA remains unmethylated in infected Vero cells

Weber S, Hakobyan A, Zakaryan H, Dörfler W (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Epigenomics Future Medicine

Book Volume: 10

Pages Range: 289-299

Journal Issue: 3

DOI: 10.2217/epi-2017-0131

Abstract

Aim: Sequence-specific CpG methylation of eukaryotic promoters is an important epigenetic signal for long-term gene silencing. We have now studied the methylation status of African swine fever virus (ASFV) DNA at various times after infection of Vero cells in culture. Methods & results: ASFV DNA was detectable throughout the infection cycle and was found unmethylated in productively infected Vero cells as documented by bisulfite sequencing of 13 viral DNA segments. Conclusion: ASFV DNA does not become de novo methylated in the course of infection in selected segments spread across the entire genome. Thus DNA methylation does not interfere with ASFV genome transcription. Lack of de novo methylation has previously been observed for free intracellular viral DNA in cells permissively infected with human adenoviruses, with human papillomaviruses and others.

Authors with CRIS profile

Stefanie Weber Department of Medicine 3 – Rheumatology and Immunology

Involved external institutions

National Academy of Sciences US United States (USA) (US)

How to cite

APA:

Weber, S., Hakobyan, A., Zakaryan, H., & Dörfler, W. (2018). Intracellular African swine fever virus DNA remains unmethylated in infected Vero cells. Epigenomics, 10(3), 289-299. https://doi.org/10.2217/epi-2017-0131

MLA:

Weber, Stefanie, et al. "Intracellular African swine fever virus DNA remains unmethylated in infected Vero cells." Epigenomics 10.3 (2018): 289-299.

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