Evaluation of adenovirus 19a as a novel vector for mucosal vaccination against influenza A viruses

Lapuente D, Ruzsics Z, Thirion C, Tenbusch M (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Vaccine Elsevier

Book Volume: 36

Pages Range: 2712-2720

Journal Issue: 19

DOI: 10.1016/j.vaccine.2018.02.075

Abstract

Since preexisting immunity and enhanced infection rates in a clinical trial of an HIV vaccine have raised some concerns on adenovirus (Ad) serotype 5-based vaccines, we evaluated the subgroup D adenovirus serotype Ad19a for its suitability as novel viral vector vaccine against mucosal infections. In BALB/c mice, we compared the immunogenicity and efficacy of E1/E3-deleted Ad19a vectors encoding the influenza A virus (IAV)-derived antigens hemagglutinin (HA) and nucleoprotein (NP) to the most commonly used Ad5 vectors. The adenoviral vectors were applied intranasally and induced detectable antigen-specific T cell responses in the lung and in the spleen as well as robust antibody responses. A prior DNA immunization significantly improved the immunogenicity of both vectors and resulted in full protection against a lethal infection with a heterologous H3N2 virus. Nevertheless, the Ad5-based vectors were slightly superior in reducing viral replication in the lung which corresponded to higher NP-specific T cell responses measured in the lungs. (C) 2018 Elsevier Ltd. All rights reserved.

Authors with CRIS profile

Matthias Tenbusch Professur für Genbasierte Immunisierungsverfahren

Involved external institutions

Ruhr-Universität Bochum (RUB) DE Germany (DE) Albert-Ludwigs-Universität Freiburg DE Germany (DE) SIRION BIOTECH GmbH DE Germany (DE)

How to cite

APA:

Lapuente, D., Ruzsics, Z., Thirion, C., & Tenbusch, M. (2018). Evaluation of adenovirus 19a as a novel vector for mucosal vaccination against influenza A viruses. Vaccine, 36(19), 2712-2720. https://doi.org/10.1016/j.vaccine.2018.02.075

MLA:

Lapuente, Dennis, et al. "Evaluation of adenovirus 19a as a novel vector for mucosal vaccination against influenza A viruses." Vaccine 36.19 (2018): 2712-2720.

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