Kaposi's sarcoma-derived cell line SLK is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line.

Stürzl M, Gaus D, Dirks WG, Ganem D, Jochmann R (2013)

Publication Status: Published

Publication Type: Journal article

Publication year: 2013

Journal

International Journal of Cancer Wiley-Blackwell

Book Volume: 132

Pages Range: 1954-8

Journal Issue: 8

DOI: 10.1002/ijc.27849

Abstract

Kaposi's sarcoma (KS) is an endothelial cell-derived tumor. Investigations of the molecular mechanisms of KS pathogenesis and the identification of drugs for treatment of KS depend critically on valid cell-culture models. Two major immortalized cell lines are available for KS research. Recently, the KS cell line KS Y-1 has been shown to be cross-contaminated with the T24 urinary bladder cancer cell line (ATCC HTB-4). Here, we show by short tandem repeat profiling that the second KS cell line, SLK, is indistinguishable from the clear-cell renal-cell carcinoma cell line Caki-1. Immunocytochemical detection of cytokeratin expression confirmed the epithelial-cell origin of SLK cells. Our findings indicate that SLK cells are not of endothelial origin and should not be used in future studies as a model for KS-derived endothelial tumor cells. We suggest that in the future, more attention needs to be paid to the authenticity of cells in lines derived from human tissues.

Authors with CRIS profile

Michael Stürzl Professur für Molekulare und Experimentelle Chirurgie Dominika Gaus Professur für Molekulare und Experimentelle Chirurgie

Involved external institutions

Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures / Deutsche Sammlung von Mikroorganismen und Zellkulturen DE Germany (DE) Novartis Institutes for BioMedical Research, Inc. US United States (USA) (US)

How to cite

APA:

Stürzl, M., Gaus, D., Dirks, W.G., Ganem, D., & Jochmann, R. (2013). Kaposi's sarcoma-derived cell line SLK is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line. International Journal of Cancer, 132(8), 1954-8. https://doi.org/10.1002/ijc.27849

MLA:

Stürzl, Michael, et al. "Kaposi's sarcoma-derived cell line SLK is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line." International Journal of Cancer 132.8 (2013): 1954-8.

BibTeX: Download