High throughput screening of gene functions in mammalian cells using reversely transfected cell arrays: review and protocol.

Stürzl M, Konrad A, Sander G, Wies E, Neipel F, Naschberger E, Reipschlaeger S, Gonin-Laurent N, Horch RE, Kneser U, Hohenberger W, Erfle H, Thurau M (2008)

Publication Status: Published

Publication Type: Journal article, Review article

Publication year: 2008

Journal

Combinatorial Chemistry & High Throughput Screening Bentham Science Publishers

Book Volume: 11

Pages Range: 159-72

Journal Issue: 2

DOI: 10.2174/138620708783744499

Abstract

Reversely transfected cell microarrays (RTCM) have been introduced as a method for parallel high throughput analysis of gene functions in mammalian cells. Hundreds to thousands of different recombinant DNA or RNA molecules can be transfected into different cell clusters at the same time on a single glass slide with this method. This allows either the simultaneous overexpression or--by using the recently developed RNA interference (RNAi) techniques--knockdown of a huge number of target genes. A growing number of sophisticated detection systems have been established to determine quantitatively the effects of the transfected molecules on the cell phenotype. Several different cell types have been successfully used for this procedure. This review summarizes the presently available knowledge on this technique and provides a laboratory protocol.

Authors with CRIS profile

Michael Stürzl Professur für Molekulare und Experimentelle Chirurgie Frank Neipel Institute of Clinical and Molecular Virology Elisabeth Naschberger Medizinische Fakultät Raymund Horch Professur für Plastische Chirurgie und Handchirurgie Werner Hohenberger Department of Surgery

Involved external institutions

Ruprecht-Karls-Universität Heidelberg DE Germany (DE)

How to cite

APA:

Stürzl, M., Konrad, A., Sander, G., Wies, E., Neipel, F., Naschberger, E.,... Thurau, M. (2008). High throughput screening of gene functions in mammalian cells using reversely transfected cell arrays: review and protocol. Combinatorial Chemistry & High Throughput Screening, 11(2), 159-72. https://doi.org/10.2174/138620708783744499

MLA:

Stürzl, Michael, et al. "High throughput screening of gene functions in mammalian cells using reversely transfected cell arrays: review and protocol." Combinatorial Chemistry & High Throughput Screening 11.2 (2008): 159-72.

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