Mitochondrial DNA recombination in Brassica campestris
Manchekar M, Scissum-Gunn KD, Hammett LA, Backert S, Nielsen BL (2009)
Publication Type: Journal article
Publication year: 2009
Journal
Book Volume: 177
Pages Range: 629-635
Journal Issue: 6
DOI: 10.1016/j.plantsci.2009.08.002
Abstract
The structure of the mitochondrial genome in plants is unclear, but appears to consist of mostly linear DNA with some other structures, including branched molecules and subgenomic circles. Mitochondrial DNA (mtDNA) recombination was analyzed in Brassica campestris, which has one of the smallest mitochondrial genomes (218 kb) in higher plants. Field-inversion gel electrophoresis (FIGE) separated mtDNA into discrete populations that each represents the entire genome. Electron microscopy revealed large, mostly linear molecules trapped in the wells, slower migrating populations with mostly linear DNA and a low level of circular and networked mtDNA molecules of 10-140 kbp, and a fast migrating population of 10-50 kbp linear mtDNA. Some smaller than genome size circular molecules and circles with tails were observed, and may represent recombination or rolling circle replication intermediates. Hybridization of end-labeled mtDNA suggests there may be specific ends (or recombination hotspots) for some linear molecules. Analysis of mtDNA enriched by BND-cellulose and separated by two-dimensional agarose gel electrophoresis shows the presence of complex recombination structures and the presence of significant single-stranded regions in mtDNA. These findings provide further evidence that DNA recombination contributes to the complex structure of mtDNA in plants. © 2009 Elsevier Ireland Ltd. All rights reserved.
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APA:
Manchekar, M., Scissum-Gunn, K.D., Hammett, L.A., Backert, S., & Nielsen, B.L. (2009). Mitochondrial DNA recombination in Brassica campestris. Plant Science, 177(6), 629-635. https://dx.doi.org/10.1016/j.plantsci.2009.08.002
MLA:
Manchekar, Medha, et al. "Mitochondrial DNA recombination in Brassica campestris." Plant Science 177.6 (2009): 629-635.
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