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Specific identification of Campylobacter fetus by PCR targeting variable regions of the 16S rDNA

Oyarzabal OA, Wesley IV, Harmon KM, Schroeder-Tucker LC, Barbaree JM, Lauerman LH, Backert S, Conner DE (1997)

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Publication Type: Journal article

Publication year: 1997

Journal

Book Volume: 58

Pages Range: 61-71

Journal Issue: 1

DOI: 10.1016/S0378-1135(97)00148-X

Abstract

Campylobacter fetus is recognized as a human and animal pathogen. The isolation and differentiation of C. fetus in diagnostic laboratories is hindered by its relatively slow growth and lack of distinguishing biochemical characteristics. We developed a fast, reliable PCR assay that specifically amplifies a 554-bp segment of the 16S rDNA from C. fetus. Fifty-two ATCC reference strains and 255 bacterial field isolates comprising the genera Campylobacter, Arcobacter, Helicobacter, Escherichia, Listeria, Salmonella, and Wolinella were evaluated using this PCR protocol. Only, C. fetus strains were amplified. Sequence analysis of amplicons from ATCC and field strains of C. fetus confirmed the presence of the target DNA fragment. The detection limit of the technique was 5.9 x 10³ CFU/ml. This PCR assay can yield reliable detection of C fetus within 3 h after isolation of presumptive colonies on agar plates.

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APA:

Oyarzabal, O.A., Wesley, I.V., Harmon, K.M., Schroeder-Tucker, L.C., Barbaree, J.M., Lauerman, L.H.,... Conner, D.E. (1997). Specific identification of Campylobacter fetus by PCR targeting variable regions of the 16S rDNA. Veterinary Microbiology, 58(1), 61-71. https://dx.doi.org/10.1016/S0378-1135(97)00148-X

MLA:

Oyarzabal, Omar A., et al. "Specific identification of Campylobacter fetus by PCR targeting variable regions of the 16S rDNA." Veterinary Microbiology 58.1 (1997): 61-71.

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