Expanding crystallization tools for nucleic acid complexes using U1A protein variants
Rosenbach H, Victor J, Borggraefe J, Biehl R, Steger G, Etzkorn M, Span I (2020)
Publication Type: Journal article
Publication year: 2020
Journal
Book Volume: 210
Article Number: 107480
Journal Issue: 2
DOI: 10.1016/j.jsb.2020.107480
Abstract
The major bottlenecks in structure elucidation of nucleic acids are crystallization and phasing. Co-crystallization with proteins is a straight forward approach to overcome these challenges. The human RNA-binding protein U1A has previously been established as crystallization module, however, the absence of UV-active residues and the predetermined architecture in the asymmetric unit constitute clear limitations of the U1A system. Here, we report three crystal structures of tryptophan-containing U1A variants, which expand the crystallization toolbox for nucleic acids. Analysis of the structures complemented by SAXS, NMR spectroscopy, and optical spectroscopy allow for insights into the potential of the U1A variants to serve as crystallization modules for nucleic acids. In addition, we report a fast and efficient protocol for crystallization of RNA by soaking and present a fluorescence-based approach for detecting RNA-binding in crystallo. Our results provide a new tool set for the crystallization of RNA and RNA:DNA complexes.
Authors with CRIS profile
Involved external institutions
Heinrich-Heine-Universität Düsseldorf
Germany (DE)
Forschungszentrum Jülich / Research Centre Jülich (FZJ)
Germany (DE)
Germany (DE)
Forschungszentrum Jülich / Research Centre Jülich (FZJ)
Germany (DE)
How to cite
APA:
Rosenbach, H., Victor, J., Borggraefe, J., Biehl, R., Steger, G., Etzkorn, M., & Span, I. (2020). Expanding crystallization tools for nucleic acid complexes using U1A protein variants. Journal of Structural Biology, 210(2). https://doi.org/10.1016/j.jsb.2020.107480
MLA:
Rosenbach, Hannah, et al. "Expanding crystallization tools for nucleic acid complexes using U1A protein variants." Journal of Structural Biology 210.2 (2020).
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