Nanosensors-Assisted quantitative analysis of biochemical processes in droplets

Belyaev D, Schuett J, Ibarlucea B, Rim T, Baraban L, Cuniberti G (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Micromachines MDPI

Book Volume: 11

Article Number: 138

Journal Issue: 2

DOI: 10.3390/mi11020138

Abstract

Here, we present a miniaturized lab-on-a-chip detecting system for an all-electric and label-free analysis of the emulsion droplets incorporating the nanoscopic silicon nanowires-based field-effect transistors (FETs). We specifically focus on the analysis of β-galactosidase e.g., activity, which is an important enzyme of the glycolysis metabolic pathway. Furthermore, the efficiency of the synthesis and action of β-galactosidase can be one of the markers for several diseases, e.g., cancer, hyper/hypoglycemia, cell senescence, or other disruptions in cell functioning. We measure the reaction and reaction kinetics-associated shift of the source-to-drain current Isd in the system, which is caused by the change of the ionic strength of the microenvironment. With these results, we demonstrate that the ion-sensitive FETs are able to sense the interior of the aqueous reactors; thus, the conjunction of miniature nanosensors and droplet-based microfluidic systems conceptually opens a new route toward a sensitive, optics-less analysis of biochemical processes.

Involved external institutions

Technische Universität Dresden

Germany (DE) Pohang University of Science and Technology (POSTECH)

Korea, Republic of (KR)

How to cite

APA:

Belyaev, D., Schuett, J., Ibarlucea, B., Rim, T., Baraban, L., & Cuniberti, G. (2020). Nanosensors-Assisted quantitative analysis of biochemical processes in droplets. Micromachines, 11(2). https://doi.org/10.3390/mi11020138

MLA:

Belyaev, Dmitry, et al. "Nanosensors-Assisted quantitative analysis of biochemical processes in droplets." Micromachines 11.2 (2020).

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