Trnp1 organizes diverse nuclear membrane-less compartments in neural stem cells.

Esgleas M, Falk S, Forne I, Thiry M, Najas S, Zhang S, Mas-Sanchez A, Geerlof A, Niessing D, Wang Z, Imhof A, Goetz M (2020)

Publication Type: Journal article

Publication year: 2020

Journal

EMBO Journal EMBO Press

Book Volume: 39

Journal Issue: 16

DOI: 10.15252/embj.2019103373

Abstract

TMF1-regulated nuclear protein 1 (Trnp1) has been shown to exert potent roles in neural development affecting neural stem cell self-renewal and brain folding, but its molecular function in the nucleus is still unknown. Here, we show that Trnp1 is a low complexity protein with the capacity to phase separate. Trnp1 interacts with factors located in several nuclear membrane-less organelles, the nucleolus, nuclear speckles, and condensed chromatin. Importantly, Trnp1 co-regulates the architecture and function of these nuclear compartments in vitro and in the developing brain in vivo. Deletion of a highly conserved region in the N-terminal intrinsic disordered region abolishes the capacity of Trnp1 to regulate nucleoli and heterochromatin size, proliferation, and M-phase length; decreases the capacity to phase separate; and abrogates most of Trnp1 protein interactions. Thus, we identified Trnp1 as a novel regulator of several nuclear membrane-less compartments, a function important to maintain cells in a self-renewing proliferative state.

Involved external institutions

Ludwig-Maximilians-Universität (LMU) DE Germany (DE) University of Chinese Academy of Sciences CN China (CN) University of Liège (ULg) / Université de Liège BE Belgium (BE) Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE)

How to cite

APA:

Esgleas, M., Falk, S., Forne, I., Thiry, M., Najas, S., Zhang, S.,... Goetz, M. (2020). Trnp1 organizes diverse nuclear membrane-less compartments in neural stem cells. EMBO Journal, 39(16). https://dx.doi.org/10.15252/embj.2019103373

MLA:

Esgleas, Miriam, et al. "Trnp1 organizes diverse nuclear membrane-less compartments in neural stem cells." EMBO Journal 39.16 (2020).

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