Mechanical spinal cord transection in larval zebrafish and subsequent whole-mount histological processing

John N, Kolb J, Wehner D (2022)

Publication Type: Journal article

Publication year: 2022

Journal

STAR Protocols Cell Press

Book Volume: 3

Article Number: 101093

Journal Issue: 1

DOI: 10.1016/j.xpro.2021.101093

Abstract

Zebrafish regenerate their spinal cord after injury, both at larval and adult stages. Larval zebrafish have emerged as a powerful model system to study spinal cord injury and regeneration due to their high optical transparency for in vivo imaging, amenability to high-throughput analysis, and rapid regeneration time. Here, we describe a protocol for the mechanical transection of the larval zebrafish spinal cord, followed by whole-mount tissue processing for in situ hybridization and immunohistochemistry to elucidate principles of regeneration. For complete details on the use and execution of this protocol, please refer to Wehner et al. (2017) and Tsata et al. (2021).

Authors with CRIS profile

Nora John
Julia Kolb

Involved external institutions

Max-Planck-Institut für die Physik des Lichts (MPL) / Max Planck Institute for the Science of Light DE Germany (DE)

How to cite

APA:

John, N., Kolb, J., & Wehner, D. (2022). Mechanical spinal cord transection in larval zebrafish and subsequent whole-mount histological processing. STAR Protocols, 3(1). https://doi.org/10.1016/j.xpro.2021.101093

MLA:

John, Nora, Julia Kolb, and Daniel Wehner. "Mechanical spinal cord transection in larval zebrafish and subsequent whole-mount histological processing." STAR Protocols 3.1 (2022).

BibTeX: Download