Preparation of Human Myocardial Tissue for Long-term Cultivation

Hamers J, Sen P, Merkus D, Seidel T, Lu K, Dendorfer A (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Journal of Visualized Experiments MYJoVE Corporation

Book Volume: 2022

Article Number: e63964

Journal Issue: 184

DOI: 10.3791/63964

Abstract

Cardiomyocyte cultivation has seen a vast number of developments, ranging from two-dimensional (2D) cell cultivation to iPSC derived organoids. In 2019, an ex vivo way to cultivate myocardial slices obtained from human heart samples was demonstrated, while approaching in vivo condition of myocardial contraction. These samples originate mostly from heart transplantations or left-ventricular assist device placements. Using a vibratome and a specially developed cultivation system, 300 µm thick slices are placed between a fixed and a spring wire, allowing for stable and reproducible cultivation for several weeks. During cultivation, the slices are continuously stimulated according to individual settings. Contractions can be displayed and recorded in real-time, and pharmacological agents can be readily applied. User-defined stimulation protocols can be scheduled and performed to assess vital contraction parameters like post-pause-potentiation, stimulation threshold, force-frequency relation, and refractory period. Furthermore, the system enables a variable pre-and afterload setting for a more physiological cultivation. Here, we present a step-by-step guide on how to generate a successful long-term cultivation of human left ventricular myocardial slices, using a commercial biomimetic cultivation solution.

Authors with CRIS profile

Thomas Seidel Professur für Herz-Kreislaufphysiologie

Involved external institutions

Klinikum der Universität München DE Germany (DE)

How to cite

APA:

Hamers, J., Sen, P., Merkus, D., Seidel, T., Lu, K., & Dendorfer, A. (2022). Preparation of Human Myocardial Tissue for Long-term Cultivation. Journal of Visualized Experiments, 2022(184). https://doi.org/10.3791/63964

MLA:

Hamers, Jules, et al. "Preparation of Human Myocardial Tissue for Long-term Cultivation." Journal of Visualized Experiments 2022.184 (2022).

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