Precise Correction of Heterozygous SHOX2 Mutations in hiPSCs Derived from Patients with Atrial Fibrillation via Genome Editing and Sib Selection

Sumer SA, Hoffmann S, Laue S, Campbell B, Raedecke K, Frajs V, Clauss S, Kaab S, Janssen JWG, Jauch A, Laugwitz KL, Dorn T, Moretti A, Rappold GA (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Stem Cell Reports Elsevier

Book Volume: 15

Pages Range: 999-1013

Journal Issue: 4

DOI: 10.1016/j.stemcr.2020.08.015

Abstract

In this study, Sumer, Hoffmann, et al., developed a strategy for the isolation of extremely rare hiPSC clones, suitable for scarless correction of heterozygous mutations by random enrichment of precisely edited cells and their detection via allele quantification. This strategy facilitates hiPSC-based gene correction regardless of the gene-editing approach.

Involved external institutions

Ruprecht-Karls-Universität Heidelberg DE Germany (DE) Technische Universität München (TUM) DE Germany (DE) Deutsches Zentrum für Herz-Kreislauf-Forschung (DZHK e.V.) DE Germany (DE)

How to cite

APA:

Sumer, S.A., Hoffmann, S., Laue, S., Campbell, B., Raedecke, K., Frajs, V.,... Rappold, G.A. (2020). Precise Correction of Heterozygous SHOX2 Mutations in hiPSCs Derived from Patients with Atrial Fibrillation via Genome Editing and Sib Selection. Stem Cell Reports, 15(4), 999-1013. https://doi.org/10.1016/j.stemcr.2020.08.015

MLA:

Sumer, Simon Alexander, et al. "Precise Correction of Heterozygous SHOX2 Mutations in hiPSCs Derived from Patients with Atrial Fibrillation via Genome Editing and Sib Selection." Stem Cell Reports 15.4 (2020): 999-1013.

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