Live imaging of adult neural stem cell behavior in the intact and injured zebrafish brain
Barbosa JS, Sanchez-Gonzalez R, Di Giaimo R, Baumgart EV, Theis FJ, Goetz M, Ninkovic J (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 348
Pages Range: 789-793
Journal Issue: 6236
Abstract
Adult neural stem cells are the source for restoring injured brain tissue. We used repetitive imaging to follow single stem cells in the intact and injured adult zebrafish telencephalon in vivo and found that neurons are generated by both direct conversions of stem cells into postmitotic neurons and via intermediate progenitors amplifying the neuronal output. We observed an imbalance of direct conversion consuming the stem cells and asymmetric and symmetric self-renewing divisions, leading to depletion of stem cells over time. After brain injury, neuronal progenitors are recruited to the injury site. These progenitors are generated by symmetric divisions that deplete the pool of stem cells, a mode of neurogenesis absent in the intact telencephalon. Our analysis revealed changes in the behavior of stem cells underlying generation of additional neurons during regeneration.
Involved external institutions
Germany (DE)How to cite
APA:
Barbosa, J.S., Sanchez-Gonzalez, R., Di Giaimo, R., Baumgart, E.V., Theis, F.J., Goetz, M., & Ninkovic, J. (2015). Live imaging of adult neural stem cell behavior in the intact and injured zebrafish brain. Science, 348(6236), 789-793. https://doi.org/10.1126/science.aaa2729
MLA:
Barbosa, Joana S., et al. "Live imaging of adult neural stem cell behavior in the intact and injured zebrafish brain." Science 348.6236 (2015): 789-793.
BibTeX: Download