Vaccinia virus-mediated melanin production allows MR and optoacoustic deep tissue imaging and laser-induced thermotherapy of cancer

Stritzker J, Kirscher L, Scadeng M, Deliolanis NC, Morscher S, Symvoulidis P, Schaefer K, Zhang Q, Buckel L, Hess M, Donat U, Bradley WG, Ntziachristos V, Szalay AA (2013)

Publication Type: Journal article

Publication year: 2013

Journal

Proceedings of the National Academy of Sciences of the United States of America National Academy of Sciences

Book Volume: 110

Pages Range: 3316-3320

Journal Issue: 9

DOI: 10.1073/pnas.1216916110

Abstract

We reported earlier the delivery of antiangiogenic single chain antibodies by using oncolytic vaccinia virus strains to enhance their therapeutic efficacy. Here, we provide evidence that gene-evoked production of melanin can be used as a therapeutic and diagnostic mediator, as exemplified by insertion of only one or two genes into the genome of an oncolytic vaccinia virus strain.We found that produced melanin is an excellent reporter for optical imaging without addition of substrate.Melanin production also facilitated deep tissue optoacoustic imaging as well asMRI. In addition,melanin was shown to be a suitable target for laser-induced thermotherapy and enhanced oncolytic viral therapy. In conclusion, melanin as a mediator for thermotherapy and reporter for different imaging modalities may soon become a versatile alternative to replace fluorescent proteins also in other biological systems. After ongoing extensive preclinical studies, melanin overproducing oncolytic virus strainsmight be used in clinical trials in patients with cancer.

Involved external institutions

Genelux US United States (USA) (US) University of California, San Diego US United States (USA) (US) Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE) Julius-Maximilians-Universität Würzburg DE Germany (DE)

How to cite

APA:

Stritzker, J., Kirscher, L., Scadeng, M., Deliolanis, N.C., Morscher, S., Symvoulidis, P.,... Szalay, A.A. (2013). Vaccinia virus-mediated melanin production allows MR and optoacoustic deep tissue imaging and laser-induced thermotherapy of cancer. Proceedings of the National Academy of Sciences of the United States of America, 110(9), 3316-3320. https://dx.doi.org/10.1073/pnas.1216916110

MLA:

Stritzker, Jochen, et al. "Vaccinia virus-mediated melanin production allows MR and optoacoustic deep tissue imaging and laser-induced thermotherapy of cancer." Proceedings of the National Academy of Sciences of the United States of America 110.9 (2013): 3316-3320.

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