Brominated skeletal components of the marine demosponges, Aplysina cavernicola and Ianthella basta: Analytical and biochemical investigations
Kunze K, Niemann H, Ueberlein S, Schulze R, Ehrlich H, Brunner E, Proksch P, Van Pee KH (2013)
Publication Type: Journal article
Publication year: 2013
Journal
Book Volume: 11
Pages Range: 1271-1287
Journal Issue: 4
DOI: 10.3390/md11041271
Abstract
Demosponges possess a skeleton made of a composite material with various organic constituents and/or siliceous spicules. Chitin is an integral part of the skeleton of different sponges of the order Verongida. Moreover, sponges of the order Verongida, such as Aplysina cavernicola or Ianthella basta, are well-known for the biosynthesis of brominated tyrosine derivates, characteristic bioactive natural products. It has been unknown so far whether these compounds are exclusively present in the cellular matrix or whether they may also be incorporated into the chitin-based skeletons. In the present study, we therefore examined the skeletons of A. cavernicola and I. basta with respect to the presence of bromotyrosine metabolites. The chitin-based-skeletons isolated from these sponges indeed contain significant amounts of brominated compounds, which are not easily extractable from the skeletons by common solvents, such as MeOH, as shown by HPLC analyses in combination with NMR and IR spectroscopic measurements. Quantitative potentiometric analyses confirm that the skeleton-associated bromine mainly withstands the MeOH-based extraction. This observation suggests that the respective, but yet unidentified, brominated compounds are strongly bound to the sponge skeletons, possibly by covalent bonding. Moreover, gene fragments of halogenases suggested to be responsible for the incorporation of bromine into organic molecules could be amplified from DNA isolated from sponge samples enriched for sponge-associated bacteria. © 2013 by the authors licensee MDPI.
Involved external institutions
Technische Universität Dresden
Germany (DE)
Heinrich-Heine-Universität Düsseldorf
Germany (DE)
Technische Universität Bergakademie Freiberg
Germany (DE)
Germany (DE)
Heinrich-Heine-Universität Düsseldorf
Germany (DE)
Technische Universität Bergakademie Freiberg
Germany (DE)
How to cite
APA:
Kunze, K., Niemann, H., Ueberlein, S., Schulze, R., Ehrlich, H., Brunner, E.,... Van Pee, K.-H. (2013). Brominated skeletal components of the marine demosponges, Aplysina cavernicola and Ianthella basta: Analytical and biochemical investigations. Marine Drugs, 11(4), 1271-1287. https://doi.org/10.3390/md11041271
MLA:
Kunze, Kurt, et al. "Brominated skeletal components of the marine demosponges, Aplysina cavernicola and Ianthella basta: Analytical and biochemical investigations." Marine Drugs 11.4 (2013): 1271-1287.
BibTeX: Download