Design of Laser Activated Antimicrobial Porous Tricalcium Phosphate-Hydroxyapatite Scaffolds for Orthopedic Applications
Filipov E, Yildiz R, Dikovska A, Sotelo L, Soma T, Avdeev G, Terziyska P, Christiansen S, Leriche A, Fernandes MH, Daskalova A (2024)
Publication Type: Journal article
Publication year: 2024
Journal
Book Volume: 15
Article Number: 36
Journal Issue: 2
DOI: 10.3390/jfb15020036
Abstract
The field of bone tissue engineering is steadily being improved by novel experimental approaches. Nevertheless, microbial adhesion after scaffold implantation remains a limitation that could lead to the impairment of the regeneration process, or scaffold rejection. The present study introduces a methodology that employs laser-based strategies for the development of antimicrobial interfaces on tricalcium phosphate–hydroxyapatite (TCP-HA) scaffolds. The outer surfaces of the ceramic scaffolds with inner porosity were structured using a femtosecond laser (λ = 800 nm; τ = 70 fs) for developing micropatterns and altering local surface roughness. The pulsed laser deposition of ZnO was used for the subsequent functionalization of both laser-structured and unmodified surfaces. The impact of the fs irradiation was investigated by Raman spectroscopy and X-ray diffraction. The effects of the ZnO-layered ceramic surfaces on initial bacterial adherence were assessed by culturing Staphylococcus aureus on both functionalized and non-functionalized scaffolds. Bacterial metabolic activity and morphology were monitored via the Resazurin assay and microscopic approaches. The presence of ZnO evidently decreased the metabolic activity of bacteria and led to impaired cell morphology. The results from this study have led to the conclusion that the combination of fs laser-structured surface topography and ZnO could yield a potential antimicrobial interface for implants in bone tissue engineering.
Involved external institutions
Institute of Electronics “Academician Emil Djakov” (BAS IE) / Институт по електроника "Академик Емил Джаков"
Bulgaria (BG)
Université polytechnique des Hauts-de-France (UPHF, UVHC)
France (FR)
Innovations-Institut für Nanotechnologie und korrelative Mikroskopie e.V. (INAM) / Institute for Nanotechnology and Correlative Microscopy
Germany (DE)
Institute of Physical Chemistry "Rostislaw Kaischew" (BAS IPC) / Институт по физикохимия "Академик Ростислав Каишев"
Bulgaria (BG)
Georgi Nadjakov Institute of Solid State Physics (BAS ISSP) / Георги Наджаков Институт по физика на твърдото
Bulgaria (BG)
Universidade do Porto
Portugal (PT)
Bulgaria (BG)
Université polytechnique des Hauts-de-France (UPHF, UVHC)
France (FR)
Innovations-Institut für Nanotechnologie und korrelative Mikroskopie e.V. (INAM) / Institute for Nanotechnology and Correlative Microscopy
Germany (DE)
Institute of Physical Chemistry "Rostislaw Kaischew" (BAS IPC) / Институт по физикохимия "Академик Ростислав Каишев"
Bulgaria (BG)
Georgi Nadjakov Institute of Solid State Physics (BAS ISSP) / Георги Наджаков Институт по физика на твърдото
Bulgaria (BG)
Universidade do Porto
Portugal (PT)
How to cite
APA:
Filipov, E., Yildiz, R., Dikovska, A., Sotelo, L., Soma, T., Avdeev, G.,... Daskalova, A. (2024). Design of Laser Activated Antimicrobial Porous Tricalcium Phosphate-Hydroxyapatite Scaffolds for Orthopedic Applications. Journal of Functional Biomaterials, 15(2). https://doi.org/10.3390/jfb15020036
MLA:
Filipov, Emil, et al. "Design of Laser Activated Antimicrobial Porous Tricalcium Phosphate-Hydroxyapatite Scaffolds for Orthopedic Applications." Journal of Functional Biomaterials 15.2 (2024).
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