PEG matrix enables cell-mediated local BMP-2 gene delivery and increased bone formation in a porcine critical size defect model of craniofacial bone regeneration

Wehrhan F, Amann KU, Molenberg A, Lutz R, Neukam FW, Schlegel KA (2012)

Publication Language: English

Publication Type: Journal article, Original article

Publication year: 2012

Journal

Clinical Oral Implants Research Wiley-Blackwell

Book Volume: 23

Pages Range: 805-813

Journal Issue: 7

DOI: 10.1111/j.1600-0501.2011.02223.x

Abstract

Purpose: This study addressed the suitability of a polyethylene glycol (PEG) matrix as scaffold for cell-mediated local BMP-2 gene transfer in a calvarial critical size defect (CSD) model. Materials and methods: PEG matrix (degradation time 10 days) and PEG membrane (degradation time 120 days) were used in the pig calvarial model. Cylindrical (1 × 1 cm) CSD (9 per animal; 20 animals) were filled with: (i) HA/TCP, covered by PEG membrane (group 1); (ii) HA/TCP, mixed with PEG matrix (group 2); and (iii) HA/TCP mixed with BMP-2 transfected osteoblasts and PEG matrix (group 3). BMP-2/4 gene transfer: liposomal in vitro transfection of BMP-2/V5-tag fusion-protein. Quantitative histomorphometry (toluidine blue staining) after 2, 4 and 12 weeks assessed bone formation. Semiquantitative immunohistochemistry estimated the expression of BMP-2 and V5-tag. Results: Group 3 showed significantly higher new bone formation than groups 1, 2 at 4 (P < 0.05) and 12 (P < 0.02) weeks. BMP-2-V5-tag was detected for 4 weeks. BMP-2 expression in group 3 was higher compared to all other groups after 2 and 4 (P < 0.02) weeks. Conclusions: The PEG matrix serves as scaffold for cell-mediated BMP-2 gene delivery in guided bone regeneration facilitating cell survival and protein synthesis for at least 4 weeks. Local BMP-2 gene delivery by PEG matrix-embedded cells leads to increased bone formation during critical size defect regeneration. © 2011 John Wiley & Sons A/S.

Authors with CRIS profile

Falk Wehrhan Department of Oral and Cranio-Maxillofacial Surgery Kerstin Ute Amann Professur für Nephropathologie Rainer Lutz Department of Oral and Cranio-Maxillofacial Surgery Friedrich Wilhelm Neukam Lehrstuhl für Zahnmedizin, insbesondere Mund-, Kiefer-, und Gesichtschirurgie (MKG)

Involved external institutions

Straumann Holding AG CH Switzerland (CH)

How to cite

APA:

Wehrhan, F., Amann, K.U., Molenberg, A., Lutz, R., Neukam, F.W., & Schlegel, K.A. (2012). PEG matrix enables cell-mediated local BMP-2 gene delivery and increased bone formation in a porcine critical size defect model of craniofacial bone regeneration. Clinical Oral Implants Research, 23(7), 805-813. https://doi.org/10.1111/j.1600-0501.2011.02223.x

MLA:

Wehrhan, Falk, et al. "PEG matrix enables cell-mediated local BMP-2 gene delivery and increased bone formation in a porcine critical size defect model of craniofacial bone regeneration." Clinical Oral Implants Research 23.7 (2012): 805-813.

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