Improved diagnosis of systemic lupus erythematosus with human-derived double-stranded DNA antigen
Li X, Tang H, Chen C, Niu Q, Zhou Z, Zheng Y, Liu Y, Munoz LE, Herrmann M, Wu P, Zhao Y (2025)
Publication Type: Journal article
Publication year: 2025
Journal
Book Volume: 267
Article Number: 116809
DOI: 10.1016/j.bios.2024.116809
Abstract
Anti-double-stranded DNA antibodies (anti-dsDNA) serve as a crucial serological indicator for systemic lupus erythematosus (SLE). Chemiluminescent immunoassay (CIA) is mainly used in clinical diagnosis of SLE, but suffers from low specificity, partially because the use of dsDNA antigens of varied sources in current CIA kits that sometimes led to controversial results. On the basis that anti-dsDNA in healthy individuals tend to selectively bind with dsDNA originating from pathogens, whereas pathogenic anti-dsDNA in SLE patients bind all forms of dsDNA, here we proposed the use of dsDNA fragment derived from human genome as antigen (synthesized via PCR using the human genomic DNA as the template). A magnetic bead-based immunofluorescence assay (IFA) was thus developed for SLE diagnosis, which exhibited improved sensitivity and specificity over CIA using the WHO reference reagent (15/174) as standard. For clinical serum sample analysis (n = 590), IFA exhibited an accuracy of 71.9% that was higher than CIA (65.3%). Crucially, the IFA results exhibited stronger correlations with the activity of SLE, renal involvement, and its prognosis. Besides the improved clinical diagnosis, the proposed IFA also holds great promise in assay standardization due to the high homogeneity of the synthetic dsDNA.
Authors with CRIS profile
Luis Munoz Becerra
Department of Medicine 3 – Rheumatology and Immunology
Martin Herrmann
Department of Medicine 3 – Rheumatology and Immunology
Additional Organisation(s)
Involved external institutions
West China Medical Center of Sichuan University / 四川大学华西医学中心
China (CN)
Sichuan University (SCU) / 四川大学
China (CN)
China (CN)
Sichuan University (SCU) / 四川大学
China (CN)
How to cite
APA:
Li, X., Tang, H., Chen, C., Niu, Q., Zhou, Z., Zheng, Y.,... Zhao, Y. (2025). Improved diagnosis of systemic lupus erythematosus with human-derived double-stranded DNA antigen. Biosensors and Bioelectronics, 267. https://doi.org/10.1016/j.bios.2024.116809
MLA:
Li, Xianming, et al. "Improved diagnosis of systemic lupus erythematosus with human-derived double-stranded DNA antigen." Biosensors and Bioelectronics 267 (2025).
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