Unrevealing the inhibitory effect of Zn on presumptive Lactobacillus sp. RJ1: A Microscopic observation

Tanoé YR, Chauhan J, Telang S, Sharma RK (2024)

Publication Type: Journal article

Publication year: 2024

Journal

Acta Microscopica Committee of Societies for Electron Microscopy (CIASEM)

Book Volume: 33

Pages Range: 21-28

Journal Issue: 2

Abstract

The study aims to evaluate the effect of Zn on a bacterial strain, which was isolated from the curd and biochemically characterized to be presumptive Lactobacillus sp. RJ1. Influence of varying concentrations of ZnCl2 on the growth of isolated Lactobacillus sp. RJ1 was evaluated. Scanning electron microscopy, facilitated by a JEOL JSM-7610FPlus FESEM, provides detailed observations on biofilm formation. Elemental dispersion analysis of X-ray (EDX) provided elemental compositions of the sample surface. The research work further explored the interaction between the bacteria and cotton cloth fabric, which served as a surface for bacterial attachment. The findings validated Lactobacillus sp. RJ1's ability to form a well-structured biofilm and confirm the suitability of cotton cloth as a material for such experiments. Furthermore, the study uncovers the presence of different shapes of nanostructures on the surface of cotton threads primarily governed by the initial concentrati ons of ZnCl2. At the 0.1M concentration of ZnCl2 needle-shaped nanostructures ranging from 200 to 480nm in length were observed on the fiber surface. It was further observed that Lactobacillus sp. RJ1 could not form biofilm in the presence of ZnCl2. Thus, it is suggested to use Zn resistant probiotic strain when co-administrating to treat intestinal dysenteries.

Involved external institutions

Manipal University Jaipur IN India (IN) Chhatrapati Shahu Ji Maharaj University IN India (IN)

How to cite

APA:

Tanoé, Y.R., Chauhan, J., Telang, S., & Sharma, R.K. (2024). Unrevealing the inhibitory effect of Zn on presumptive Lactobacillus sp. RJ1: A Microscopic observation. Acta Microscopica, 33(2), 21-28.

MLA:

Tanoé, Yaba Rosette, et al. "Unrevealing the inhibitory effect of Zn on presumptive Lactobacillus sp. RJ1: A Microscopic observation." Acta Microscopica 33.2 (2024): 21-28.

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