Transient decrease in circulating dendritic cell precursors after acute stroke: Potential recruitment into the brain
Yilmaz A, Fuchs T, Dietel B, Altendorf R, Cicha I, Stumpf C, Schellinger PD, Blümcke I, Schwab S, Daniel W, Garlichs C, Kollmar R (2010)
Publication Type: Journal article
Publication year: 2010
Journal
Book Volume: 118
Pages Range: 147-157
Journal Issue: 2
DOI: 10.1042/CS20090154
Abstract
The role of DCs (dendritic cells) as potent mediators of inflammation has not been sufficiently investigated in stroke. Therefore, in the present study, circulating mDCPs (myeloid DC precursors), pDCPs (plasmacytoid DCPs) and tDCPs (total DCPs) were analysed by flow cytometry in (i) healthy controls (n = 29), (ii) patients with ACI-S (asymptomatic cerebral infarction stenosis; n = 46), (iii) patients with TIA (transient ischaemic attack; n = 39), (iv) patients with AIS (acute ischaemic stroke; n = 73), and (v) patients with AHS (acute haemorrhagic stroke; n = 31). The NIHSS (National Institutes of Health Stroke Scale) and infarction size on a CT (computer tomography) scan were evaluated after stroke. In a patient subgroup, post-mortem immunohistochemical brain analyses were performed to detect mDCs (CD209), pDCs (CD123), T-cells (CD3) and HLA-DR. In AIS and AHS, the numbers of circulating mDCPs (P < 0.005), pDCPs (P < 0.005) and tDCPs (P < 0.001) were significantly reduced. A significant inverse correlation was found between the NIHSS and circulating DCPs (P < 0.02), as well as between hsCRP (high-sensitivity C-reactive protein) and circulating DCPs (P < 0.001). Patients with large stroke sizes on a CT scan had significantly lower numbers of mDCPs (P = 0.007), pDCPs (P = 0.05) and tDCPs (P = 0.01) than those with smaller stroke sizes. Follow-up analysis showed a significant recovery of circulating DCPs in the first few days after stroke. In the infarcted brain, a dense infiltration of mDCs co-localized with T-cells, single pDCs and high HLA-DR expression were observed. In conclusion, acute stroke leads to a decrease in circulating DCPs. Potentially, circulating DCPs are recruited from the blood into the infarcted brain and probably trigger cerebral immune reactions there. © The Authors Journal compilation © 2010 Biochemical Society.
Authors with CRIS profile
Barbara Dietel
Graduiertenzentrum der FAU
Christian Stumpf
Department of Medicine 2 – Cardiology and Angiology
Ingmar Blümcke
Lehrstuhl für Neuropathologie
Stefan Schwab
Lehrstuhl für Neurologie
Werner Daniel
Medizinische Fakultät
Christoph Garlichs
Medizinische Fakultät
Rainer Kollmar
Medizinische Fakultät
Involved external institutions
Germany (DE)How to cite
APA:
Yilmaz, A., Fuchs, T., Dietel, B., Altendorf, R., Cicha, I., Stumpf, C.,... Kollmar, R. (2010). Transient decrease in circulating dendritic cell precursors after acute stroke: Potential recruitment into the brain. Clinical Science, 118(2), 147-157. https://doi.org/10.1042/CS20090154
MLA:
Yilmaz, Atilla, et al. "Transient decrease in circulating dendritic cell precursors after acute stroke: Potential recruitment into the brain." Clinical Science 118.2 (2010): 147-157.
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