Guided Multispectral Optoacoustic Tomography for 3D Imaging of the Murine Colon

Knieling F, Brown EL, Eckstein M, Thoma OM, Wachter F, Mandelbaum H, Ludwig P, Claßen M, Oraiopoulou ME, Rother U, Neurath M, Wölfle J, Waldner M, Friedrich O, Knieling F, Bohndiek SE, Regensburger A (2025)

Publication Type: Journal article

Publication year: 2025

Journal

Advanced Science Wiley Open Access

DOI: 10.1002/advs.202413434

Abstract

Multispectral optoacoustic tomography is a promising medical imaging modality that combines light and sound to provide molecular imaging information at depths of several centimeters, based on the optical absorption of endogenous chromophores, such as hemoglobin. Assessment of inflammatory bowel disease has emerged as a promising clinical application of optoacoustic tomography. In this context, preclinical studies in animal models are essential to identify novel disease-specific imaging biomarkers and understand findings from emerging clinical pilot studies, however to-date, these studies have been limited by the precise identification of the bowel wall. Herein, a transrectal-absorber guide is applied, serving as a high-contrast landmark for 3D optoacoustic tomography of the colon. This study shows that guided multispectral optoacoustic tomography is able to measure changes in blood oxygenation status over the course of acute, chemically-induced colitis in mice and correlates with standard disease activity scores. This novel approach depicts intestinal hemoglobin composition non-invasively during murine inflammation. These results underscore the potential for optoacoustic imaging in translational inflammatory bowel disease research.

Authors with CRIS profile

Ferdinand Knieling Department of Paediatrics and Adolescent Medicine Markus Eckstein Institute of Pathology Oana-Maria Thoma Department of Medicine 1 – Gastroenterology, Pneumology and Endocrinology Henriette Mandelbaum Department of Paediatrics and Adolescent Medicine Merle Claßen Lehrstuhl für Kinder- und Jugendmedizin Ulrich Rother Department of Vascular Surgery Markus Neurath Lehrstuhl für Innere Medizin I (Medizin 1) Joachim Wölfle Lehrstuhl für Kinder- und Jugendmedizin Maximilian Waldner Lehrstuhl für Innere Medizin I (Medizin 1) Oliver Friedrich Lehrstuhl für Medizinische Biotechnologie (MBT) Adrian Regensburger Department of Paediatrics and Adolescent Medicine

Involved external institutions

University of Cambridge GB United Kingdom (GB)

How to cite

APA:

Knieling, F., Brown, E.L., Eckstein, M., Thoma, O.-M., Wachter, F., Mandelbaum, H.,... Regensburger, A. (2025). Guided Multispectral Optoacoustic Tomography for 3D Imaging of the Murine Colon. Advanced Science. https://doi.org/10.1002/advs.202413434

MLA:

Knieling, Ferdinand, et al. "Guided Multispectral Optoacoustic Tomography for 3D Imaging of the Murine Colon." Advanced Science (2025).

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