Исследование проникающей способности квантовых точек InP/ZnSe/ZnS 650 в структуры переднего сегмента глаза в эксперименте ex vivo

Ponomarev VO, Kazaikin VN, Tkachenko KA, Kovalenko EG, Vokhmintsev AS, Weinstein IA, Zhdanov AE, Kulyabin M (2024)

Publication Type: Journal article

Publication year: 2024

Journal

Fyodorov Journal of Ophthalmic Surgery Russian Society of Ophthalmologists

Pages Range: 84-96

Journal Issue: 4

DOI: 10.25276/0235-4160-2024-4-84-96

Abstract

Purpose. To study the penetration ability of InP/ZnSe/ZnS 650 QDs into the structures of the anterior segment of the eye in an ex vivo experiment. Material and methods. First part of the study assessed the penetration ability of QD into the anterior chamber of the eye while mechanically reducing the thickness of the corneal tissue. In cadaver eyes (n=18), epithelium removal of the cornea was performed with subsequent layer-by-layer sections to a depth of 130, 390 and 780 µm. After installing the exposure baths, cadaver eyes were divided into 3 groups depending on the exposure time to a 10% InP/ZnSe/ZnS 650 QD solution, including a control group. After completion of the exposure, the surface of the cornea was washed with physiological solution, followed by taking its contents in a volume of 0.2 ml for spectrophotometric and luminescent analysis. The second part of the study assessed the penetration ability of QD into the layers of the cornea depending on the solution concentration and exposure time. In cadaver eyes (n=18), epithelium removal of the cornea was performed. After installing the exposure baths, cadaver eyes were divided into 3 groups depending on the exposure time of the QD solutions, including a control group and into 3 subgroups depending on the concentration (10, 0.1, 0.01%) of the InP/ZnSe QD solution/ZnS 650. After completion of the exposure phase, the corneal surface was washed with physiological solution. Subsequently, to study the flaps of each of the three groups, sections of the corneal flaps were made to a depth of 130, 260, 390 µm and were subsequently transferred to a glass slide for analysis. Results. When analyzing the studied samples of aqueous humour of the anterior chamber according to spectrophotometry and luminescence spectrometry, no reliable data were obtained for the penetration of QD into the anterior chamber of the eye. Also, when analyzing corneal sections, it was found that QDs penetrate deep into the corneal tissue, regardless of the concentration of the QD solution and exposure time. Conclusion. Based on spectrophotometry and luminescence spectrometry data, it was established that InP/ZnSe/ZnS 650 QDs do not penetrate into the anterior chamber with mechanical changes in the thickness of the corneal tissue. InP/ZnSe/ZnS 650 QDs penetrate the corneal tissue to a depth of 390 µm; a direct correlation has been established with exposure time and solution concentration.

Authors with CRIS profile

Mikhail Kulyabin Lehrstuhl für Informatik 5 (Mustererkennung)

Involved external institutions

Intersectoral Research and Technology Complex Eye Microsurgery / S. Fyodorov Eye Microsurgery Federal State Institution / Межотраслевой научно-технический комплекс «Микрохирургия глаза» RU Russian Federation (RU) Ural Federal University B.N. Yeltsin RU Russian Federation (RU)

How to cite

APA:

Ponomarev, V.O., Kazaikin, V.N., Tkachenko, K.A., Kovalenko, E.G., Vokhmintsev, A.S., Weinstein, I.A.,... Kulyabin, M. (2024). Исследование проникающей способности квантовых точек InP/ZnSe/ZnS 650 в структуры переднего сегмента глаза в эксперименте ex vivo. Fyodorov Journal of Ophthalmic Surgery, 4, 84-96. https://doi.org/10.25276/0235-4160-2024-4-84-96

MLA:

Ponomarev, Vyacheslav O., et al. "Исследование проникающей способности квантовых точек InP/ZnSe/ZnS 650 в структуры переднего сегмента глаза в эксперименте ex vivo." Fyodorov Journal of Ophthalmic Surgery 4 (2024): 84-96.

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