Expression of stem cell factor receptor c-kit in human nontumoral and tumoral hepatic cells
Mansuroglu T, Baumhoer D, Dudas J, Haller F, Cameron S, Lorf T, Füzesi L, Ramadori G (2009)
Publication Type: Journal article
Publication year: 2009
Journal
Book Volume: 21
Pages Range: 1206-1211
Journal Issue: 10
DOI: 10.1097/MEG.0b013e328317f4ef
Abstract
INTRODUCTION: The aim of this study was to examine and compare the stem cell factor receptor c-kit expression in hepatocellular carcinoma (HCC) with the corresponding peritumoral tissue. To confirm the immunohistochemical results in the investigated HCC tissues, HCC cell lines were analyzed for c-kit expression. METHODS: Expression of c-kit (SCF receptor) has been evaluated in 72 HCC and in the corresponding surrounding nontumorous tissue. Additionally, immunohistochemical analysis reverse transcription-polymerase chain reaction was also used to examine the mRNA expression of c-kit protooncogene in tumor homogenates. Furthermore, three HCC cell lines (HUH-7, HepG2, and SK-Hep1) were used for gene-expression analysis of c-kit mRNA. RESULTS: C-kit expression was detected immunohistochemically in 70% of HCC with different degrees of intensity. Moreover, c-kit expression could also be found in about 90% of the corresponding peritumoral noncirrhotic as well as in cirrhotic liver tissues. C-kit mRNA was detectable in 83% of HCC and in 75% of the corresponding peritumoral noncirrhotic as well as in 100% of corresponding peritumoral cirrhotic samples. In addition, two of the three HCC cell lines (HUH-7 and SK-Hep1) showed a well detectable PCR-product for c-kit. CONCLUSION: Hepatocytes express the c-kit receptor at different grade of intensity under normal and altered pathological conditions. The presence of c-kit on HCC cell lines supports the assumption that SCF might play a role in the regulation of proliferative activity of tumorous and nontumorous hepatic cells. © 2009 Lippincott Williams & Wilkins, Inc.
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Germany (DE)How to cite
APA:
Mansuroglu, T., Baumhoer, D., Dudas, J., Haller, F., Cameron, S., Lorf, T.,... Ramadori, G. (2009). Expression of stem cell factor receptor c-kit in human nontumoral and tumoral hepatic cells. European Journal of Gastroenterology & Hepatology, 21(10), 1206-1211. https://doi.org/10.1097/MEG.0b013e328317f4ef
MLA:
Mansuroglu, Tümen, et al. "Expression of stem cell factor receptor c-kit in human nontumoral and tumoral hepatic cells." European Journal of Gastroenterology & Hepatology 21.10 (2009): 1206-1211.
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