Validation of the Chick Chorioallantoic Membrane (CAM) Model for Biocompatibility Analysis of Biomaterials in the Context of the 3R‐cascade: A Pilot Study

Barbeck M, Hübner K, Burkhardt K, Andreeva T, Krastev R, Schnettler R, Stojanovic S, Najman S, Jung O, Schneider-Stock R (2025)

Publication Type: Journal article

Publication year: 2025

Journal

In Vivo International Institute of Anticancer Research (IIAR)

Book Volume: 39

Pages Range: 1740-1750

Journal Issue: 3

DOI: 10.21873/invivo.13977

Abstract

Background/Aim: The present study aimed to compare the tissue responses to biomaterials in the chick chorioallantoic membrane (CAM) model with those from the subcutaneous implantation model in rats at an early time point. It was especially investigated whether histopathological scoring according to DIN EN ISO 10993‑6 is also possible after biomaterial implantation using the CAM model and to what extent the values differ from the data obtained from small animal experiments. Materials and Methods: Implantation of a xenogeneic bone substitute using the CAM model for 24 h and subcutaneous implantation model in rats up to 10 days post implantation were conducted. Standardized histological and histopathological methods were used to apply for histopathological scoring according to DIN EN ISO 10993‑6. Results: The histological analysis as well as the histo‑pathological scoring revealed that the tissue responses to the xenogeneic bone substitute were completely comparable in both organisms with no visible or statistical differences. Conclusion: We suggest that bioincompatible biomaterials can already be sorted out in the context of THE preclinical in vivo test phase. Such pre‑testing before the required small animal tests might clearly contribute to the 3R‑concept to reduce the number of animals (REDUCE).

Authors with CRIS profile

Kerstin Hübner Institute of Pathology Regine Schneider-Stock Professur für Experimentelle Tumorpathologie

Involved external institutions

Universitätsmedizin Rostock DE Germany (DE) Hochschule Reutlingen DE Germany (DE) Justus-Liebig-Universität Gießen DE Germany (DE) University of Nis / Univerzitet u Nišu / Универзитет у Нишу RS Serbia (RS)

How to cite

APA:

Barbeck, M., Hübner, K., Burkhardt, K., Andreeva, T., Krastev, R., Schnettler, R.,... Schneider-Stock, R. (2025). Validation of the Chick Chorioallantoic Membrane (CAM) Model for Biocompatibility Analysis of Biomaterials in the Context of the 3R‐cascade: A Pilot Study. In Vivo, 39(3), 1740-1750. https://doi.org/10.21873/invivo.13977

MLA:

Barbeck, Mike, et al. "Validation of the Chick Chorioallantoic Membrane (CAM) Model for Biocompatibility Analysis of Biomaterials in the Context of the 3R‐cascade: A Pilot Study." In Vivo 39.3 (2025): 1740-1750.

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