Nonlinear dynamics of femtosecond laser interaction with the central nervous system in zebrafish

Jun S, Herbst A, Scheffter K, John N, Kolb J, Wehner D, Fattahi H (2025)

Publication Type: Conference contribution

Publication year: 2025

Journal

Publisher: SPIE

Book Volume: 13317

Conference Proceedings Title: Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Event location: San Francisco, CA, USA

ISBN: 9781510683822

DOI: 10.1117/12.3039763

Abstract

Understanding the photodamage processes driven by the complex nonlinear interactions of femtosecond laser pulses within the second tissue transparency window is pivotal for developing novel label-free microscopy techniques. In this research, we identify two distinct cavitation mechanisms produced by 1030 nm femtosecond pulses in the zebrafish central nervous system. The damage remains highly localized for irradiation at lower laser repetition rates, resulting from plasma-induced ablation and localized heating. In contrast, higher laser repetition rates lead to more extensive damage mediated by plasma-induced photochemical reactions. We also investigate how fluorescence labels, involving both linear and nonlinear absorption pathways, influence the dynamics of the photodamage. To confirm our findings, we analyzed cell death and tissue responses to laser-induced injury, including the activation of fibroblasts and the recruitment of immune cells.

Authors with CRIS profile

Involved external institutions

Max-Planck-Institut für die Physik des Lichts (MPL) / Max Planck Institute for the Science of Light Germany (DE)

How to cite

APA:

Jun, S., Herbst, A., Scheffter, K., John, N., Kolb, J., Wehner, D., & Fattahi, H. (2025). Nonlinear dynamics of femtosecond laser interaction with the central nervous system in zebrafish. In Norbert Linz, Joel N. Bixler, Alex J. Walsh (Eds.), Progress in Biomedical Optics and Imaging - Proceedings of SPIE. San Francisco, CA, USA: SPIE.

MLA:

Jun, Soyeon, et al. "Nonlinear dynamics of femtosecond laser interaction with the central nervous system in zebrafish." Proceedings of the Optical Interactions with Tissue and Cells XXXVI 2025, San Francisco, CA, USA Ed. Norbert Linz, Joel N. Bixler, Alex J. Walsh, SPIE, 2025.

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