Myoblast and ADSC coculture on conductive highly aligned nanofiber scaffolds for human skeletal muscle tissue engineering

Shi X, Cai A, Arkudas A, Horch RE, Jabeen S, Schubert DW, Weisbach V, Kratzer S, Stumpfe M, Mengen L (2025)

Publication Type: Journal article

Publication year: 2025

Journal

Biomedical Materials Institute of Physics

Book Volume: 21

Journal Issue: 1

DOI: 10.1088/1748-605X/ae1c08

Abstract

Conductive materials play a crucial role in enhancing functional performance in muscle tissue engineering. This study investigates the impact of the conductive polymer polyaniline (PANi) in Polycaprolactone (PCL)-collagen Type I (PCL-collagen I) nanofiber scaffolds designed to support the coculture of human adipose-derived stem cells (ADSCs) and myoblasts (Mbs). The effect of varying PANi concentrations (0%, 2%, 4%, 6%) in PCL-collagen I nanofiber scaffolds was evaluated concerning the cell alignment, differentiation and gene expression of cocultured Mbs and ADSC. Nanofiber scaffolds with different PANi concentrations were analyzed. Acetic acid was used as a non-toxic and biocompatible solvent for electrospinning the nanofibers.In vitroexperiments involved a 1:1 coculture of Mbs and ADSCs for up to 28 d on the scaffolds. The cell viability, differentiation and myotube morphology were assessed using live-dead-assay, CCK-8-assay, immunofluorescence staining and gene expression analysis. Scaffolds with 2% and 4% PANi showed a higher percentage of live cells compared to the control at both 7 and 28 d. The nanofibers with 2%, 4% and 6% PANi concentration showed promising results in terms of cell differentiation and myotube morphology. After 14 d, the scaffolds with 4% PANi showed superior cell differentiation with strong myotube alignment along the nanofibers. At higher PANi concentrations (6%), only the myotube width increased significantly, whereas 4% PANi resulted in a markedly higher myotube number. PCL-collagen I nanofibers incorporating PANi enhance myoblast alignment and differentiation compared to the control group, showing promise for muscle tissue engineering applications. The non-toxic solvent makes the nanofibers suitable for translational purposes. Furtherin vivostudies are needed to explore the full impact on cellular function and regeneration.

Authors with CRIS profile

Xiu Shi Department of Plastic and Hand Surgery Aijia Cai Department of Plastic and Hand Surgery Andreas Arkudas Department of Plastic and Hand Surgery Raymund Horch Professur für Plastische Chirurgie und Handchirurgie Sehrish Jabeen Lehrstuhl für Werkstoffwissenschaften (Polymerwerkstoffe) (LSP) Dirk Schubert Lehrstuhl für Werkstoffwissenschaften (Polymerwerkstoffe) (LSP) Volker Günter Weisbach Department of Transfusion Medicine and Haemostaseology Simon Kratzer Department of Plastic and Hand Surgery Maximilian Stumpfe Department of Plastic and Hand Surgery Lilly Mengen Department of Plastic and Hand Surgery

How to cite

APA:

Shi, X., Cai, A., Arkudas, A., Horch, R.E., Jabeen, S., Schubert, D.W.,... Mengen, L. (2025). Myoblast and ADSC coculture on conductive highly aligned nanofiber scaffolds for human skeletal muscle tissue engineering. Biomedical Materials, 21(1). https://doi.org/10.1088/1748-605X/ae1c08

MLA:

Shi, Xiu, et al. "Myoblast and ADSC coculture on conductive highly aligned nanofiber scaffolds for human skeletal muscle tissue engineering." Biomedical Materials 21.1 (2025).

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